Research on high-speed super-resolution in vivo 3D imaging method by utilizing advanced laser light technology

The purpose of this subject is to realize biological super-resolution imaging that contributes to connectomics research. By taking full advantages of novel optical laser technologies, we will develop a system that can be applied for multiple fluorescence wavelengths and realize high-speed three-dimensional image acquisition. Finally, by utilizing stimulated emission suppression to break the spatial resolution limited by classical diffraction of light and by combining it with a multiphoton excitation microscope excellent for the deep observation of the living body, we will realize super-resolution imaging of a model animal's living brain.