Elucidating circuit function via multiplexed activity trace labeling and all-optical interrogation

We previously engineered a next-generation, genetically encoded calcium indicator, XCaMPs, which can monitor neuronal activity at high speeds. We also combined the use of synthetic activity-dependent promoters, such as E-SARE, making it possible to visualize cell activity for long durations. Based on these results, we will design and develop novel technologies to simultaneously record multiple cell types, including excitatory and inhibitory neurons. We will develop functional imaging to visualize neuronal activities of multiple cell types at high speeds in the live brains of animals while they perform behavioral tasks. In addition, we will establish technologies that enable an all-optical interrogation based on multiplexed activity trace recording.